Vaccination either before or after SARSCoV2 infection may both confer stronger immune responses than vaccination alone, according to a new SciImmunology analysis of serum from 104 vaccinated people.
) In 96-well plates . Plates were coated with 100 μL/well of the following proteins at 1 μg/mL in PBS and incubated overnight at 4°C with rocking: SARS-CoV-2 RBD , Full-length SARS-CoV-2 spike , Nucleocapsid . Plates were washed three times with 0.05% v/v Tween-20 in PBS and blocked with 150 μL/well 5% nonfat dry milk powder in wash buffer at room temperature of approximately 20°C for 1 hour with rocking.
8 × 10-fold dilutions were prepared in dilution media and 30 μL/well of diluted virus was incubated with the cells for 1 hour at TCC before further addition of Opti-MEM, 2% FBS, 1% methylcellulose and incubation for 24 hours at TCC. Plates were then fixed by soaking in 4% formaldehyde in PBS for 1 hour then removing from BSL-3 following institutional biosafety protocols. Cells were permeabilized in 0.1% bovine serum albumin and 0.
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