Nature research paper: Teixobactin kills bacteria by a two-pronged attack on the cell envelope
, pH 7.4) three times. For fixing the cells, they were resuspended in a 4% formalin and allowed to incubate at 37 °C for 10 min. They were washed once again with the buffer and resuspended in 200 μl of buffer. Of the stained and fixed cells, 50 μl were then pipetted onto the agarose beds and covered with coverslip. The bacterial coverslips were imaged using Zeiss LSM 700 with a ×100/1.2 NA oil objective lens. Lys-teixobactin was excited using a 488-nm laser.
The HS-AFM images were acquired in amplitude modulation tapping mode in liquid using a high-speed atomic force microscope . Short cantilevers with a nominal spring constant of 0.15 N mwere used . A minimal imaging force was applied by using a small set-point amplitude of 0.8 nm . The HS-AFM results showing the assembly of teixobactin filaments and membrane deformation were obtained from imaging of supported lipid bilayers on mica.
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