Genetic 'hitchhikers' can be directed using CRISPR ncstate
Escherichia coli
BL21 cells. For transposition experiments involving a pDonor construct with a donor transposon of approximately 1 kb in size, pEffector and pDonor constructs were co-transformed into chemically competent cells by using 50 ng of each plasmid. For transposition experiments involving pDonor constructs with a donor transposon of ∼10 kb in size, 50 ng of each plasmid was co-transformed via electroporation into electrocompetent cells using the manufacturer's recommended settings.
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