First structure of key COVID enzyme at human body temperature SciReports
HEPES pH 7.0, 3–5% DMSO and incubating at a temperature of ∼298 K, crystals were seen after 2–6 d.Individual crystals were harvested using 10 µm MicroMesh loops . For cryogenic temperature, crystals were cryocooled by the traditional practice of plunging into liquid nitrogen. For non-cryogenic temperatures at ambient humidity, crystals were coated with Paratone-N oil, then mounted on the goniometer for data collection.
X-ray diffraction data were collected at the National Synchrotron Light Source II beamline 17-ID-2 using an X-ray beam of energy 12.66 keV, corresponding to a wavelength of 0.9793 Å; a horizontal-bounce Si111 double-crystal monochromator; and an EIGER X 16M pixel array detector . The temperature at the sample goniometer was controlled using a Cryostream 800 . For the 298 K, 99.5% data set, RH was controlled with an HC-LAB Humidity Controller .
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