Abscisic acid (ABA) is a key plant hormone produced in response to abiotic stress such as drought and salt. It is mainly synthesized in roots and vascular tissues and transported to specific sites to exert physiological functions. Several ABA transporters have been identified; however, the molecular mechanism underlying specific binding and cross-membrane transport of ABA remains unknown.
transporter ABCG25 from Arabidopsis . The study was mainly conducted by Prof. Zhang Peng's group from Center for Excellence in Molecular Plant Sciences of the Chinese Academy of Sciences, collaborating with Prof. Chen Zhenguo's group from Fudan University.
AtABCG25 protein was expressed and purified through heterologous expression, and different 3D structural conformations of AtABCG25, including inward-facing apo conformation (AtABCG25
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