Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice
). Cells were mounted onto slides using Mounting Media containing DAPI. A minimum of three images per well were captured using a spinning disk confocal microscope ). Data representing two independent experiments are presented.The plasma membrane protein extraction kit was used to separate the plasma membrane fraction from other cellular membranes according to the manufacturer’s recommended protocol. Data representing two independent experiments are presented.
Plasma concentration was measured from blood collected using standard and established protocols.SW48 cells were subcutaneously injected into the flanks of 6–8-week old athymic nude female mice and tumor growth was monitored with caliper measurement. Once the mean tumor reached a certain volume , animals were randomized into different treatment groups of five animals per group. 249C for in vivo use was formulated in 0.5% methylcellulose and dosed i.p. .
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