Multi-omics resources for targeted agronomic improvement of pigmented rice - Nature Food

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Multi-omics resources for targeted agronomic improvement of pigmented rice - Nature Food
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We screened the metabolic profiles of 24 BR, 35 RR and 4 BrR varieties. The mature grains were ground in liquid nitrogen and lyophilized for 30 h, after which the samples were prepared by Metabolon Inc. Several recovery standards were added prior to the extraction process. The samples were extracted with 80% methanol under vigorous shaking for 2 min .

Raw UPLC–MS/MS data were extracted and filtered to remove those representing system artefacts, misassignments, redundancy and background noise. Peaks and compounds were identified by comparison to library entries of the purified standards, and the peaks were quantified as area-under-the-curve detector ion counts; Welch’s two-sampleWe quantified the metal ion content of the same 63 rice varieties.

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