Human ureteric bud organoids recapitulate branching morphogenesis and differentiate into functional collecting duct cell types
, From day 7, the UB organoids showed a gradual decline in the tip marker, which is more broadly expressed in the stalk components, was maintained at relatively stable levels. n = 3 independent biological replicates per timepoint. Scale bars, 50 μm . Column and error bars represent mean and standard deviation, respectively.
Extended Data Fig. 6 UB progenitor cells participate in niche interactions in chimeric explants but not with hPSC-derived metanephric cells., Cells from induced UB organoids and metanephric kidney organoids were dissociated at day 7, mixed and reaggregated, and cultured as spheres in suspension. Within two days the GATA3/KRT8-expressing UB cells had formed an epithelial network in the inner portion of the organoid.
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