Peptides that bind MHC Class I are readily identified with a programmable, high-throughput method
A lentiviral pHAGE vector with a cytomegalovirus promoter plus an EF1α promoter driving EGFP–P2A-Purowas used as the backbone. The vector was digested with PstI and AgeI to excise the EF1α promoter, and the Gibson assembly method was used to insert a gBlock encoding a codon-optimized mouse mammary tumor virus gp70 signal peptide, filler region flanked by BsmBI sites and an IRES element.
Evaluating all top ESP predictions on the proteome. Of all ESPv1 proteome predictions, those with peptides with a binding probability of <0.05 or <0.1 were chosen: 84,580 peptides for HLA-A*02, HLA-B*08 and HLA-B*57 and 92,000 for HLA*03.
Indonesia Berita Terbaru, Indonesia Berita utama
Similar News:Anda juga dapat membaca berita serupa dengan ini yang kami kumpulkan dari sumber berita lain.
Peptides, Explained: How a Skin Care Supercharger Became Beauty’s Big BuzzwordThis class of ingredients comes with science-backed performance, though not all peptides are alike. Here, a primer on a little-understood ingredient, plus 15 products that address everything from wrinkles to dark spots.
Baca lebih lajut »
Silvana Estrada on Mercedes Sosa: 'Her voice Is a Force of Nature'For Rolling Stone's annual Icons & Influences series, silvanaestradab talks about the deep influence Argentine activist and folk legend Mercedes Sosa had on her music. 'You can hear where she comes from, where she’s going, and who she’s singing for.'
Baca lebih lajut »
UK, BioNTech test mRNA against cancer - Nature BiotechnologyIn Brief: NHSEngland and GenomicsEngland partner with BioNTech_Group to fast-track up to 10,000 patients into clinical trials of mRNA immunotherapies to treat cancer
Baca lebih lajut »
Deconvolution of clinical variance in CAR-T cell pharmacology and response - Nature BiotechnologyA model of T cell differentiation control deconvolutes biological mechanisms underlying clinical variance.
Baca lebih lajut »
Interstrand crosslinking of homologous repair template DNA enhances gene editing in human cells - Nature BiotechnologyInterstrand crosslinking of homologous repair template DNA enhances gene editing in human cells - mcdbucsb
Baca lebih lajut »
Proximity-induced superconductivity in epitaxial topological insulator/graphene/gallium heterostructures - Nature MaterialsThis work reports on the synthesis and proximity-induced superconductivity in a topological insulator-based, thin-film heterostructure towards the development of a scalable material platform that could potentially support robust quantum computing.
Baca lebih lajut »