Extending fluorescence anisotropy to large complexes using reversibly switchable proteins
. Taken together, our
methods to characterize the properties of immature and mature VLPs labeled with rsEGFP2., Anisotropy curves measured with method 2 from HIV-1 labeled with rsEGFP2 detected immature and mature particles in time-resolved mode with increased sensitivity. Single-particle average anisotropy values were extracted from small regions of interest including one VLP ., Images of mature and immature virions labeled with rsEGFP2 extracted from
method 2 anisotropy values for immature and mature particles measured on RESOLFT images ., Representative fluorescence images in grayscale of Arc and mutant and fluorescence anisotropy curves .
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