In the core of the cell: New insights into the utilization of nanotechnology-based drugs NatureComms
Detergent Removal Spin Columns according to the manufacturer’s instructions. After SDS removal, 25 µg of protein per sample were precipitated with ProteoExtract Protein Precipitation Kit , following the manufacturer’s instructions. The precipitated proteins were isolated by centrifugation at 10,000 ×for 10 min at RT and two wash steps. The supernatant was finally removed and the pellet dried for 5–10 min.
Digested samples were diluted with LC–MS grade water containing 0.1% formic acid . To perform an absolute quantification according to a previously established protocolHI3 Ecoli Standard . The samples were measured by a nanoACQUITY UPLC system, installed with a C18 nanoACQUITY trap column and a C18 analytical reversed-phase column . The mobile phases used for separation consisted of 0.1% formic acid in water and 0.1% formic acid in acetonitrile .
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