Nature research paper: An airway-to-brain sensory pathway mediates influenza-induced sickness
) or diphtheria toxin supplemented with 0.05% Fast Green FCF Dye using a sharply pulled glass pipette attached to a Nanoject Injector . AAV-cre , AAV-flex-tdTomato , AAV-GFP , and AAV-flex-AP were purchased. Successful injection was verified by Fast Green FCF Dye slowly filling the entire ganglion without leakage. Surgical wounds were closed with coated VICRYL Sutures and animals received Buprenorphine SR as an analgesic.
heparin, 1× Denhardt’s solution, 10% dextran sulfate), probe wash buffer , amplification buffer , and fluorophore-labelled HCR amplifiers were purchased from Molecular Instruments. NJP ganglia were freshly collected, mounted in OCT and cryosectioned . Tissue was post-fixed , washed , treated with 1% hydrogen peroxide , and incubated withHCR probes .
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