Nature research paper: A prebiotically plausible scenario of an RNA–peptide world
to capture the reactivity in structures that were large and hydrophobic enough to exclude water. This would then have been the transition from the non-canonical nucleoside-based RNA–peptide world to the ribosome-centred translational process that is a hallmark of all life on Earth today.The RNA donor and acceptor strands were annealed with NaCl by heating at 95 °C for 4 min, followed by cooling down slowly to room temperature.
After that, MES buffer pH 6 and NaCl were added to the oligonucleotide solution. Finally, carboxylic acid or nitrile activator/s and water were added to the solution mixture. The peptide coupling reaction was incubated at 25 °C for 24 h. The crude reaction mixtures were analysed by HPLC and MALDI-TOF mass spectrometry.The hairpin-type intermediate was diluted with MES buffer pH 6 or acetate buffer pH 4 , NaCl and water .
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