Nature research paper: A cell-type-specific error-correction signal in the posterior parietal cortex
driven by the Sst44 enhancer in two C57Bl/6J male mice at 2–3 sites spaced anterior-posterior by 0.4 mm centred on , −2.0 mm from bregma, 0.3 mm below the dura, 70 nl per site, 1 × 10GC per ml in PBS). The injection pipette was inserted through a small craniotomy and was angled at 30° from the horizontal such that the injection site was under the bone. For recordings, we removed the bone above the injection sites.
This experiment aims to test whether Sst44 cell activity is required for the mouse to execute a course correction. The outcome of these experiments is that we did not observe a difference in the mouse’s behaviour on trials in which we targeted the blue laser light to the PPC compared with interleaved trials in which we targeted the laser to control sites.
For photostimulation experiments, we included sessions in which mice performed the task with >80% correct trials. This resulted in 20 sessions across 4 mice in the PPC . These cells were independently sampled.For each cell, we computed the background-subtracted intensity for each channel by taking the mean intensity within that cell’s spatial mask and subtracting the mean intensity in a two-pixel outline around the mask. Cell types were called on a relative basis.
We pooled trials across sessions for these triggered analyses. Note that, although cells were not independent for trials from different sessions from the same mouse, the same is also true for trials within the same session. To compare more data, we therefore compiled trials across sessions, conceptually treating trials in the same and different sessions as equivalent.
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